#cell-dilution-calculator {
max-width: 500px;
margin: 20px auto;
padding: 15px;
background: #fff;
border-radius: 5px;
box-shadow: 0 1px 3px rgba(0,0,0,0.1);
font-family: -apple-system, BlinkMacSystemFont, ‘Arial’, sans-serif;
color: #333;
box-sizing: border-box;
}
h2 {
text-align: center;
margin: 0 0 15px;
font-size: 1.4rem;
line-height: 1.2;
color: #333;
}
.form-group {
margin-bottom: 15px;
position: relative;
}
label {
display: block;
margin-bottom: 5px;
font-size: 0.9rem;
font-weight: 600;
}
input[type=”number”] {
width: 100%;
padding: 8px;
border: 1px solid #ddd;
border-radius: 3px;
font-size: 0.9rem;
box-sizing: border-box;
}
input:focus {
border-color: #0073aa;
outline: none;
}
.tooltip {
position: relative;
display: inline-block;
margin-left: 5px;
}
.tooltip .tooltip-text {
visibility: hidden;
width: 160px;
background: #333;
color: #fff;
text-align: center;
border-radius: 3px;
padding: 5px;
position: absolute;
z-index: 10;
bottom: 125%;
left: 50%;
transform: translateX(-50%);
opacity: 0;
transition: opacity 0.3s;
font-size: 0.75rem;
}
.tooltip:hover .tooltip-text {
visibility: visible;
opacity: 1;
}
.button-group {
display: flex;
gap: 10px;
justify-content: center;
margin-top: 15px;
}
button {
padding: 8px 16px;
border: none;
border-radius: 3px;
cursor: pointer;
font-size: 0.9rem;
background: #0073aa;
color: #fff;
}
button:hover {
background: #005177;
}
#reset-btn {
background: #6c757d;
}
#reset-btn:hover {
background: #5a6268;
}
#result {
margin-top: 15px;
padding: 10px;
border-radius: 3px;
font-size: 0.9rem;
line-height: 1.4;
min-height: 20px;
}
#result.success {
background: #e6f4ea;
border: 1px solid #28a745;
}
#result.error {
background: #f8d7da;
border: 1px solid #dc3545;
}
.result-table {
width: 100%;
border-collapse: collapse;
margin-top: 10px;
}
.result-table th, .result-table td {
padding: 8px;
border: 1px solid #ddd;
text-align: left;
font-size: 0.85rem;
}
.result-table th {
background: #f8f9fa;
font-weight: 600;
}
noscript {
display: block;
color: #dc3545;
text-align: center;
margin-top: 10px;
font-size: 0.9rem;
}
@media (max-width: 500px) {
#cell-dilution-calculator {
margin: 10px;
padding: 10px;
}
h2 {
font-size: 1.2rem;
}
.button-group {
flex-direction: column;
}
button {
width: 100%;
}
.result-table th, .result-table td {
font-size: 0.8rem;
padding: 6px;
}
}
Volume of Stock Solution: ${initialVolume.toFixed(2)} mL
Volume of Diluent: ${diluentVolume.toFixed(2)} mL
Dilution Factor: ${dilutionFactor.toFixed(2)}-fold
Summary:
Interpretation:
${interpretation} `; resultDiv.className = ‘success’; } catch (error) { resultDiv.innerHTML = `Error: ${error.message}`; resultDiv.className = ‘error’; } } function reset() { form.reset(); resultDiv.className = ”; resultDiv.innerHTML = ‘Enter concentration and volume, then click “Calculate”.’; initialConcInput.focus(); } document.getElementById(‘calculate-btn’).addEventListener(‘click’, calculate); document.getElementById(‘reset-btn’).addEventListener(‘click’, reset); form.addEventListener(‘keypress’, function(e) { if (e.key === ‘Enter’ && e.target.tagName !== ‘BUTTON’) { e.preventDefault(); calculate(); } }); })();
Cell Dilution Calculator
Enter concentration and volume, then click “Calculate”.
JavaScript is disabled. Please enable it to use the calculator.
(function() {
if (!document.getElementById(‘cell-dilution-calculator’)) return;
const form = document.getElementById(‘calc-form’);
const initialConcInput = document.getElementById(‘initial-conc’);
const finalConcInput = document.getElementById(‘final-conc’);
const finalVolumeInput = document.getElementById(‘final-volume’);
const resultDiv = document.getElementById(‘result’);
function calculate() {
resultDiv.className = ”;
resultDiv.innerHTML = ”;
try {
const initialConc = parseFloat(initialConcInput.value);
const finalConc = parseFloat(finalConcInput.value);
const finalVolume = parseFloat(finalVolumeInput.value);
if (isNaN(initialConc) || initialConc <= 0) throw new Error('Initial concentration must be a positive number.');
if (isNaN(finalConc) || finalConc <= 0) throw new Error('Final concentration must be a positive number.');
if (isNaN(finalVolume) || finalVolume initialConc) throw new Error(‘Final concentration cannot exceed initial concentration.’);
const initialVolume = (finalConc * finalVolume) / initialConc;
const diluentVolume = finalVolume – initialVolume;
const dilutionFactor = initialConc / finalConc;
let interpretation = ”;
if (dilutionFactor < 10) {
interpretation = 'Low dilution, suitable for maintaining high cell viability.';
} else if (dilutionFactor < 100) {
interpretation = 'Moderate dilution, common for cell culture experiments.';
} else {
interpretation = 'High dilution, ensure sufficient mixing to avoid uneven distribution.';
}
resultDiv.innerHTML = `
Cell Dilution Results:Volume of Stock Solution: ${initialVolume.toFixed(2)} mL
Volume of Diluent: ${diluentVolume.toFixed(2)} mL
Dilution Factor: ${dilutionFactor.toFixed(2)}-fold
Summary:
| Parameter | Value |
|---|---|
| Initial Concentration | ${initialConc.toLocaleString()} cells/mL |
| Final Concentration | ${finalConc.toLocaleString()} cells/mL |
| Final Volume | ${finalVolume} mL |
| Stock Volume | ${initialVolume.toFixed(2)} mL |
| Diluent Volume | ${diluentVolume.toFixed(2)} mL |
Interpretation:
${interpretation} `; resultDiv.className = ‘success’; } catch (error) { resultDiv.innerHTML = `Error: ${error.message}`; resultDiv.className = ‘error’; } } function reset() { form.reset(); resultDiv.className = ”; resultDiv.innerHTML = ‘Enter concentration and volume, then click “Calculate”.’; initialConcInput.focus(); } document.getElementById(‘calculate-btn’).addEventListener(‘click’, calculate); document.getElementById(‘reset-btn’).addEventListener(‘click’, reset); form.addEventListener(‘keypress’, function(e) { if (e.key === ‘Enter’ && e.target.tagName !== ‘BUTTON’) { e.preventDefault(); calculate(); } }); })();