#ligation-calculator {
max-width: 500px;
margin: 20px auto;
padding: 15px;
background: #fff;
border-radius: 5px;
box-shadow: 0 1px 3px rgba(0,0,0,0.1);
font-family: -apple-system, BlinkMacSystemFont, ‘Arial’, sans-serif;
color: #333;
box-sizing: border-box;
}
h2 {
text-align: center;
margin: 0 0 15px;
font-size: 1.4rem;
line-height: 1.2;
color: #333;
}
p {
text-align: center;
color: #666;
font-size: 14px;
margin-bottom: 15px;
}
.form-group {
margin-bottom: 15px;
position: relative;
display: flex;
align-items: center;
gap: 10px;
}
label {
display: block;
margin-bottom: 5px;
font-size: 0.9rem;
font-weight: 600;
}
input[type=”number”], select {
padding: 8px;
border: 1px solid #ddd;
border-radius: 3px;
font-size: 0.9rem;
box-sizing: border-box;
}
input[type=”number”] {
width: 70%;
}
select {
width: 30%;
}
input:focus, select:focus {
border-color: #0073aa;
outline: none;
}
.tooltip {
position: relative;
display: inline-block;
margin-left: 5px;
}
.tooltip .tooltip-text {
visibility: hidden;
width: 160px;
background: #333;
color: #fff;
text-align: center;
border-radius: 3px;
padding: 5px;
position: absolute;
z-index: 10;
bottom: 125%;
left: 50%;
transform: translateX(-50%);
opacity: 0;
transition: opacity 0.3s;
font-size: 0.75rem;
}
.tooltip:hover .tooltip-text {
visibility: visible;
opacity: 1;
}
.button-group {
display: flex;
gap: 10px;
justify-content: center;
margin-top: 15px;
}
button {
padding: 8px 16px;
border: none;
border-radius: 3px;
cursor: pointer;
font-size: 0.9rem;
background: #0073aa;
color: #fff;
}
button:hover {
background: #005177;
}
#reset-btn {
background: #6c757d;
}
#reset-btn:hover {
background: #5a6268;
}
#result {
margin-top: 15px;
padding: 10px;
border-radius: 3px;
font-size: 0.9rem;
line-height: 1.4;
min-height: 20px;
text-align: center;
}
#result.success {
background: #e6f4ea;
border: 1px solid #28a745;
}
#result.error {
background: #f8d7da;
border: 1px solid #dc3545;
}
noscript {
display: block;
color: #dc3545;
text-align: center;
margin-top: 10px;
font-size: 0.9rem;
}
@media (max-width: 500px) {
#ligation-calculator {
margin: 10px;
padding: 10px;
}
h2 {
font-size: 1.2rem;
}
.form-group {
flex-direction: column;
align-items: stretch;
}
input[type=”number”], select {
width: 100%;
}
.button-group {
flex-direction: column;
}
button {
width: 100%;
}
}
Required Insert Mass: ${requiredInsertMass.toFixed(2)} ng`; if (requiredInsertMass < 50) { output += '
Note: It\’s recommended to use at least 50 ng of insert for better cloning success.‘; } resultDiv.innerHTML = output; resultDiv.className = ‘success’; } catch (error) { resultDiv.innerHTML = `Error: ${error.message}`; resultDiv.className = ‘error’; } } function reset() { form.reset(); resultDiv.className = ”; resultDiv.innerHTML = ‘Enter DNA lengths, vector mass, and molar ratio, then click “Calculate”.’; insertLengthInput.focus(); } document.getElementById(‘calculate-btn’).addEventListener(‘click’, calculate); document.getElementById(‘reset-btn’).addEventListener(‘click’, reset); form.addEventListener(‘keypress’, function(e) { if (e.key === ‘Enter’ && e.target.tagName !== ‘BUTTON’) { e.preventDefault(); calculate(); } }); })();
Ligation Calculator
Calculate the optimal mass of insert DNA needed for a ligation reaction based on vector DNA mass and desired molar ratio.
bp
kb
bp
kb
ng
μg
Enter DNA lengths, vector mass, and molar ratio, then click “Calculate”.
JavaScript is disabled. Please enable it to use the calculator.
(function() {
if (!document.getElementById(‘ligation-calculator’)) return;
const form = document.getElementById(‘calc-form’);
const insertLengthInput = document.getElementById(‘insert-length’);
const insertUnitSelect = document.getElementById(‘insert-unit’);
const vectorLengthInput = document.getElementById(‘vector-length’);
const vectorUnitSelect = document.getElementById(‘vector-unit’);
const vectorMassInput = document.getElementById(‘vector-mass’);
const vectorMassUnitSelect = document.getElementById(‘vector-mass-unit’);
const molarRatioInput = document.getElementById(‘molar-ratio’);
const resultDiv = document.getElementById(‘result’);
function calculate() {
resultDiv.className = ”;
resultDiv.innerHTML = ”;
try {
let insertLength = parseFloat(insertLengthInput.value);
let insertUnit = insertUnitSelect.value;
if (insertUnit === ‘kb’) insertLength *= 1000; // Convert kb to bp
let vectorLength = parseFloat(vectorLengthInput.value);
let vectorUnit = vectorUnitSelect.value;
if (vectorUnit === ‘kb’) vectorLength *= 1000; // Convert kb to bp
let vectorMass = parseFloat(vectorMassInput.value);
let vectorMassUnit = vectorMassUnitSelect.value;
if (vectorMassUnit === ‘μg’) vectorMass *= 1000; // Convert μg to ng
let molarRatio = parseFloat(molarRatioInput.value);
if (isNaN(insertLength) || insertLength <= 0) throw new Error('Insert length must be a positive number.');
if (isNaN(vectorLength) || vectorLength <= 0) throw new Error('Vector length must be a positive number.');
if (isNaN(vectorMass) || vectorMass <= 0) throw new Error('Vector mass must be a positive number.');
if (isNaN(molarRatio) || molarRatio <= 0) throw new Error('Molar ratio must be a positive number.');
const requiredInsertMass = vectorMass * molarRatio * (insertLength / vectorLength);
let output = `Result:Required Insert Mass: ${requiredInsertMass.toFixed(2)} ng`; if (requiredInsertMass < 50) { output += '
Note: It\’s recommended to use at least 50 ng of insert for better cloning success.‘; } resultDiv.innerHTML = output; resultDiv.className = ‘success’; } catch (error) { resultDiv.innerHTML = `Error: ${error.message}`; resultDiv.className = ‘error’; } } function reset() { form.reset(); resultDiv.className = ”; resultDiv.innerHTML = ‘Enter DNA lengths, vector mass, and molar ratio, then click “Calculate”.’; insertLengthInput.focus(); } document.getElementById(‘calculate-btn’).addEventListener(‘click’, calculate); document.getElementById(‘reset-btn’).addEventListener(‘click’, reset); form.addEventListener(‘keypress’, function(e) { if (e.key === ‘Enter’ && e.target.tagName !== ‘BUTTON’) { e.preventDefault(); calculate(); } }); })();