#qpcr-efficiency-calculator {
max-width: 600px;
margin: 20px auto;
padding: 15px;
background: #fff;
border-radius: 5px;
box-shadow: 0 1px 3px rgba(0,0,0,0.1);
font-family: -apple-system, BlinkMacSystemFont, ‘Arial’, sans-serif;
color: #333;
box-sizing: border-box;
}
h2 {
text-align: center;
margin: 0 0 15px;
font-size: 1.4rem;
line-height: 1.2;
color: #333;
}
p {
text-align: center;
color: #666;
font-size: 14px;
margin-bottom: 15px;
}
.form-group {
margin-bottom: 15px;
position: relative;
}
label {
display: block;
margin-bottom: 5px;
font-size: 0.9rem;
font-weight: 600;
}
input[type=”number”] {
width: 100%;
padding: 8px;
border: 1px solid #ddd;
border-radius: 3px;
font-size: 0.9rem;
box-sizing: border-box;
}
input:focus {
border-color: #0073aa;
outline: none;
}
.tooltip {
position: relative;
display: inline-block;
margin-left: 5px;
}
.tooltip .tooltip-text {
visibility: hidden;
width: 180px;
background: #333;
color: #fff;
text-align: center;
border-radius: 3px;
padding: 5px;
position: absolute;
z-index: 10;
bottom: 125%;
left: 50%;
transform: translateX(-50%);
opacity: 0;
transition: opacity 0.3s;
font-size: 0.75rem;
}
.tooltip:hover .tooltip-text {
visibility: visible;
opacity: 1;
}
.button-group {
display: flex;
gap: 10px;
justify-content: center;
margin-top: 15px;
}
button {
padding: 8px 16px;
border: none;
border-radius: 3px;
cursor: pointer;
font-size: 0.9rem;
background: #0073aa;
color: #fff;
}
button:hover {
background: #005177;
}
#reset-btn {
background: #6c757d;
}
#reset-btn:hover {
background: #5a6268;
}
#copy-btn {
background: #28a745;
}
#copy-btn:hover {
background: #218838;
}
#result {
margin-top: 15px;
padding: 10px;
border-radius: 3px;
font-size: 0.9rem;
line-height: 1.4;
min-height: 20px;
}
#result.success {
background: #e6f4ea;
border: 1px solid #28a745;
}
#result.error {
background: #f8d7da;
border: 1px solid #dc3545;
}
#standard-curve {
display: block;
margin: 10px auto;
max-width: 100%;
border: 1px solid #ddd;
}
noscript {
display: block;
color: #dc3545;
text-align: center;
margin-top: 10px;
font-size: 0.9rem;
}
@media (max-width: 600px) {
#qpcr-efficiency-calculator {
margin: 10px;
padding: 10px;
}
h2 {
font-size: 1.2rem;
}
.button-group {
flex-direction: column;
}
button {
width: 100%;
}
}
Estimated Relative Quantity: ${relativeQuantity.toExponential(2)} (based on Ct = ${ctValue})`; } drawStandardCurve(slope); resultDiv.innerHTML = ` qPCR Efficiency: ${efficiency.toFixed(2)}%
Amplification Factor: ${amplificationFactor.toFixed(3)}
Interpretation: ${interpretation}
Tips: ${tips} ${quantityEstimate}
`; resultDiv.className = ‘success’; document.getElementById(‘copy-btn’).addEventListener(‘click’, () => { const text = ` qPCR Efficiency Results Slope: ${slope} Efficiency: ${efficiency.toFixed(2)}% Amplification Factor: ${amplificationFactor.toFixed(3)} Interpretation: ${interpretation} Tips: ${tips} ${quantityEstimate ? `Estimated Relative Quantity: ${relativeQuantity.toExponential(2)} (Ct = ${ctValue})` : ”} `.trim(); navigator.clipboard.writeText(text).then(() => { alert(‘Results copied to clipboard!’); }); }); } catch (error) { resultDiv.innerHTML = `Error: ${error.message}`; resultDiv.className = ‘error’; } } function reset() { form.reset(); resultDiv.className = ”; resultDiv.innerHTML = ‘Enter the slope, then click “Calculate”.’; canvas.style.display = ‘none’; slopeInput.focus(); } document.getElementById(‘calculate-btn’).addEventListener(‘click’, calculate); document.getElementById(‘reset-btn’).addEventListener(‘click’, reset); form.addEventListener(‘keypress’, function(e) { if (e.key === ‘Enter’ && e.target.tagName !== ‘BUTTON’) { e.preventDefault(); calculate(); } }); })();
Enhanced qPCR Efficiency Calculator
Calculate qPCR efficiency with a standard curve visualization.
Enter the slope, then click “Calculate”.
JavaScript is disabled. Please enable it to use the calculator.
(function() {
if (!document.getElementById(‘qpcr-efficiency-calculator’)) return;
const form = document.getElementById(‘calc-form’);
const slopeInput = document.getElementById(‘slope’);
const ctValueInput = document.getElementById(‘ct-value’);
const resultDiv = document.getElementById(‘result’);
const canvas = document.getElementById(‘standard-curve’);
const ctx = canvas.getContext(‘2d’);
function drawStandardCurve(slope, intercept = 20) {
canvas.style.display = ‘block’;
ctx.clearRect(0, 0, canvas.width, canvas.height);
const padding = 40;
const xMax = canvas.width – padding * 2;
const yMax = canvas.height – padding * 2;
const logQuantities = [0, 1, 2, 3, 4, 5];
const ctValues = logQuantities.map(x => intercept + slope * x);
ctx.beginPath();
ctx.strokeStyle = ‘#0073aa’;
ctx.lineWidth = 2;
ctx.moveTo(padding, canvas.height – padding);
ctx.lineTo(canvas.width – padding, canvas.height – padding);
ctx.moveTo(padding, padding);
ctx.lineTo(padding, canvas.height – padding);
ctx.stroke();
ctx.font = ’12px Arial’;
ctx.fillStyle = ‘#333’;
ctx.textAlign = ‘center’;
ctx.fillText(‘Log(Quantity)’, canvas.width / 2, canvas.height – 10);
ctx.save();
ctx.translate(10, canvas.height / 2);
ctx.rotate(-Math.PI / 2);
ctx.fillText(‘Ct Value’, 0, 0);
ctx.restore();
const xScale = xMax / 5;
const yScale = yMax / (Math.max(…ctValues) – Math.min(…ctValues));
const yMin = Math.min(…ctValues);
logQuantities.forEach((x, i) => {
ctx.fillText(x.toString(), padding + x * xScale, canvas.height – padding + 15);
const y = canvas.height – padding – (ctValues[i] – yMin) * yScale;
if (i === 0) {
ctx.beginPath();
ctx.moveTo(padding + x * xScale, y);
} else {
ctx.lineTo(padding + x * xScale, y);
}
});
ctx.strokeStyle = ‘#28a745’;
ctx.stroke();
ctValues.forEach((ct, i) => {
const y = canvas.height – padding – (ct – yMin) * yScale;
ctx.beginPath();
ctx.arc(padding + i * xScale, y, 3, 0, 2 * Math.PI);
ctx.fillStyle = ‘#28a745’;
ctx.fill();
});
}
function calculate() {
resultDiv.className = ”;
resultDiv.innerHTML = ”;
canvas.style.display = ‘none’;
try {
const slope = parseFloat(slopeInput.value);
const ctValue = parseFloat(ctValueInput.value) || null;
if (isNaN(slope)) {
throw new Error(‘Slope must be a number.’);
}
if (slope >= 0) {
throw new Error(‘Slope must be negative (e.g., -3.32).’);
}
if (ctValue !== null && ctValue = 90 && efficiency <= 110) {
interpretation = 'Acceptable efficiency (90–110%). Your qPCR assay is performing well.';
tips = 'Maintain current conditions for reliable quantification.';
} else if (efficiency < 90) {
interpretation = 'Efficiency too low (110%). Possible assay issues.’;
tips = ‘Investigate primer dimers, sample inhibitors, or dilution errors.’;
}
let quantityEstimate = ”;
if (ctValue !== null) {
const relativeQuantity = Math.pow(amplificationFactor, -ctValue);
quantityEstimate = `Estimated Relative Quantity: ${relativeQuantity.toExponential(2)} (based on Ct = ${ctValue})`; } drawStandardCurve(slope); resultDiv.innerHTML = ` qPCR Efficiency: ${efficiency.toFixed(2)}%
Amplification Factor: ${amplificationFactor.toFixed(3)}
Interpretation: ${interpretation}
Tips: ${tips} ${quantityEstimate}
`; resultDiv.className = ‘success’; document.getElementById(‘copy-btn’).addEventListener(‘click’, () => { const text = ` qPCR Efficiency Results Slope: ${slope} Efficiency: ${efficiency.toFixed(2)}% Amplification Factor: ${amplificationFactor.toFixed(3)} Interpretation: ${interpretation} Tips: ${tips} ${quantityEstimate ? `Estimated Relative Quantity: ${relativeQuantity.toExponential(2)} (Ct = ${ctValue})` : ”} `.trim(); navigator.clipboard.writeText(text).then(() => { alert(‘Results copied to clipboard!’); }); }); } catch (error) { resultDiv.innerHTML = `Error: ${error.message}`; resultDiv.className = ‘error’; } } function reset() { form.reset(); resultDiv.className = ”; resultDiv.innerHTML = ‘Enter the slope, then click “Calculate”.’; canvas.style.display = ‘none’; slopeInput.focus(); } document.getElementById(‘calculate-btn’).addEventListener(‘click’, calculate); document.getElementById(‘reset-btn’).addEventListener(‘click’, reset); form.addEventListener(‘keypress’, function(e) { if (e.key === ‘Enter’ && e.target.tagName !== ‘BUTTON’) { e.preventDefault(); calculate(); } }); })();