qPCR Efficiency Calculator
Free online calculator to determine qPCR efficiency from standard curve slope. Provides efficiency percentage, amplification factor, and interpretation with visualization.
Biotechnology
Ligation Calculator
Free online ligation calculator for molecular cloning. Determines optimal insert DNA mass based on vector length, insert length, and desired molar ratio for successful ligation reactions.
DNA Concentration Calculator
Free online DNA concentration calculator. Convert A260 absorbance values to ng/μL concentrations for double-stranded DNA, single-stranded DNA, and RNA using standard conversion factors.
Cell Doubling Time Calculator
This free online calculator computes cell doubling time and number of generations from initial and final cell counts over a given time period. Essential for cell culture and microbiology work.
Cell Dilution Calculator
Free online cell dilution calculator for laboratory use. Calculate required volumes and dilution factors for cell culture experiments with precise results.
Annealing Temperature Calculator
A free online calculator to determine the optimal annealing temperature for your PCR primers, ensuring efficient and specific DNA amplification in molecular biology experiments.